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Double Digestion Fingerprinting: AFLP®-PCR amplification
Genome: Escherichia coli K12
 Reference, Map from NCBI
Amplified fragments
RE1 RE1-RE2 RE2

RE2 RE1-RE2 RE1


Restriction enzymes
Restriction enzyme 1 (RE1):
FunII,EcoRI
 G'AATT_C
Selective nucleotides: AC
Restriction enzyme 2 (RE2):
MseI,Tru1I,Tru9I
 T'TA_A
Selective nucleotides: C




Escherichia coli K12 (4639221 bp)


PositionLengthDirection



990859508RE2-RE1
101637098RE1-RE2
104700099RE1-RE2
1605044121RE2-RE1
1898343401RE1-RE2
20125231041RE2-RE1
220712156RE1-RE2
2543418175RE2-RE1
2564818745RE2-RE1
3148600382RE1-RE2
3186025503RE2-RE1
3304145404RE1-RE2
34479411002RE1-RE2
3481571261RE1-RE2
356588885RE1-RE2
35899861301RE1-RE2
4318758537RE2-RE1
4342467111RE1-RE2
4355355218RE1-RE2
443462014RE1-RE2
4613767113RE1-RE2




























































































Length of bands (21 bands)

  1301
  1041
  1002
  745
  537
  508
  503
  404
  401
  382
  261
  218
  175
  121
  113
  111
  99
  98
  85
  56
  14
Length of bands includes complete endonuclease recognition sequences.
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