In silico PCR-RFLP

For information about usage of in silico PCR amplification tool check here.

For information about usage of restriction digest tool check here.

This script will compute amplicons yielded by PCR amplification, and position of amplicons within genome and their length will be shown. In a second step, the amplicons will be digested with selected endonucleases, and length of resulting fragments will be calculated. Results from this second step is shown in a table (next to length of amplicons obtained in first step).

The data above and a simulation of bands pattern after electrophoresis are shown in the response page.