Sequence retrieval

Genome: Chloroflexus sp. Y-400-fl
Start position: 4999597
End position: 4999692
Length: 96

DNA sequence
>NC_012032, from 4999597 to 4999692 (96 bp); Chloroflexus sp. Y-400-fl TTTCTATAGGGGCGGGTTTCTATAGGGGCGGGTTTCTATAGGGGCGGGTTTCTATAGGGGCGGGTTTCTA TAGGGGCGGGTTTCTATAGGGGCGGG
Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF... 4999649-4999762	Sequence	Chy400_4047 - hypothetical protein (Protein is coded in complementary strand; from 4999762 to 4999649)