Sequence retrieval

Genome: Chloroflexus sp. Y-400-fl
Start position: 4216074
End position: 4216325
Length: 252

DNA sequence
>NC_012032, from 4216074 to 4216325 (252 bp); Chloroflexus sp. Y-400-fl ATGCAGCGGATGAACATTCTTTTGCGGGCCGGAGGCCCGCGCTCCCAGCGTCCAGGGATGGCTGGACACT TGCCGGTAACGCCGCCTGCATCCGTTTCCCCTCACCTGTCATCCTTGAACTTCCTATCTCTTATCTCCTA TCTCCTAATCTTCTATCTCCTATCTTCTATCTCCTATCTCCTACCTCCTATCTCCTACCTCCTACCTCCT CCCCCTACCCCGCCTGCTGTGGCGCAACTTCTTTCGCCGTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4216074-4216325	Sequence	Chy400_3385 - hypothetical protein
ORF... 4216288-4217802	Sequence	Chy400_3386 - EmrB/QacA subfamily drug resistance transporter (Protein is coded in complementary strand; from 4217802 to 4216288)