Sequence retrieval

Genome: Clostridium difficile CD196
Start position: 1114021
End position: 1117560
Length: 3540

DNA sequence
>NC_013315, from 1114021 to 1117560 (3540 bp); Clostridium difficile CD196 GTGAATCAGCAGATGAGACCAATTAGATTAGAACTTACAGGACTTAATAGCTATATTGATAAACAAGTTA TAGACTTTGAAAAATTAATTGAAAGAGGTTTGTTTGGGATATTTGGAACTACTGGAAGTGGAAAATCTAC AATCTTAGATGCAATAACTATTGCTATGTATGGAAATATATCCAGAAATACAAAAGAATATATAAACAGT GTGTGCGATAAGGCAATTATATCATACGAGTTTGAGATAGGAAGCAAAAACACTAGAAGAAGATACATTG TAGATAGAACCATTGCTAGAAGCAAAACAGGAACAAAGACCTCTAATGCTAGATTAATAGAAGTATTGAA TGACAATATCCAAAATGTTTTAGCTGATAAAGTTGTGGAAGTAAATGAAAAAGTAGCACAGGTTGTAGGA TTAACGGCAAATGATTTTACTAGGTCAGTAGTTTTACCACAAGACAAATTTAATGAGTTTCTAAGATTAT CACGTGCAGATAGAAGAGACATGTTAGAGAGAATTTTTAATCTTGAGAAATATGGAAGAAGTCTTGGAGA AAAAGTGAAAAAGAGAAAAAATATACAGTTACAAAATTTAAAAGATTTGAAATCAAAATTAAGTCAATAT GATGGAATTACAGAGGAAGTATACAACAATATAAACCAAGAATTAATAGAGTTAAAAAATTTGGAAAAAG ATAAGAATAATGCTTTAGACCTAGCTCAAAAATCTTATGAAGAAAGTAAAACAGTATATGAAGAACAATT AAAGTTAGAAAAAAATGAACTTAGAAAAAATGAGCTTGATTTAAAAAATAGTGAGATAAAAGAAAAGATT GCTCTTGTAGAAAATGATAGTAACGCTAAAAAGGTAGACCCATACATAAGTTCAGTTCAAAATCTAGAAA AAAAGATAGATGAAGATAGCTTTGTAGTAAGTAATTTAGAAAAGAAATTAGCAATATTAAATCAGGAGCT TGAAGTTACAAAAAATAGATATGAAAAAATAAACAAAATTAAGAATGAAGAAGTCCCTAAATTGAGTGAA GAAAAAATAAGATTGCAACAAGCAATAAAATTAGAAGAAGAGTTAGTTTTATTAGATAGAGAGTTAAAAG ATCTAAAAGAAAGTGGAATTAATTTAAATAAGACTAAAGTTGAATTAGAAAAAGTAAAGCAGGTGTCAGA GTCCAGAAAAGATGCAGTTACTAAATCTATAAAAGAAGTTGAAGGAAAGATTGATAAAGTAAATATAAGT GCAGAATTAAAACAAAAAATATTTCTAGCATATGAATACGAGAAAGATTACAACAACGTATTGGAAGAAA AAAATCAAAAGCTAAATAAACTAGAAGAAATACTCAAAGAGACAGAAAATATAAATTTAAAAGTTAGATA TATTGATAAAGATAAGAATGATGTAAATCGTAATTTAGAAAATTTATCACTTCACTTGGATGTTCTTCTT AAAAAATGTCCTGGTAAGAGTGCTGATTTACTTTTAAAATCTGAATATGTTACTGAATTAAGAAGTAAAG CAAATAATACTAAAGAAAATGAAATTAAGAAAAGCAGTATACAAGATGAATTAAAGATAATACTTGAAAG TAAGTTTAATACTGAAAGGGAAGTAAATTTACTAAATGAAAAACTTGAAAATAATAGAAAGAATAGAGAT GACCTAGAAAAAGAATTAGAAGAACTAAAGTATTTAAATTTAGCCTCTGAACTTAGACGTGAATTAAAAG AAAATATGCCTTGTCCAGTTTGTGGGTCAAAACATCATGAAAATCATAATATAACTAAGTATGATGAAAA TATATCTTTTGTCAAAGAAAAACTGGAGAAATTAGAAAAAGAAAAAATAAGTATAAGAAACAATATAGAG GAATTAAATGCTAAAGTAAGTGGGTATTTATCTATAGAAAAAATGAAGACTAAAGAACTTGAAGATGTAA AAGGAAAACTAGGGGGGATTCCATCTAGTCAATTATTAAAAAAACTTGATGAAGAACAAAGAAAACTTGC ATTGTTAAAATCTAATATTCAGGAGTGGGAGAAAGAAAAAGAGAGTACTGAAAATAAAGTAACATTAGCT AAAGAAGAAAAAAATAAAATTGAAAAAGAAGAATTAAAAATAAGAGAGAGTCTGAATAATTATAAGAAAT TAACAAGAGATTTAAACATAGAAATAGAAGGTTTAGAAAATAAATGTAAAAAACTTAAACAGGAATACCT AGGATTAAAGACGATTACTAAAGTCTCTAATCTATTGTCAAAAGTAAAAGAAATAAGGGAGAATGAAAAA GAATTAGAATTACTAAGTACTGGGCATTCAAATTTATTAAAAAATAGAGACTCACTAGATATTAAAATAA GAGAAAATGAGAGTAAACTACATGAAATAGAGATAGAATTAATAAAGGCTAGAGAATTATATGTAGAAAA GAAATTGAGTAGAGATAATAAGTATAAAGAAGTTATTAACATAACGAAAGGTGATTTAGCTAAAAATCTT TTACATAATGTTGAAGAAAATATATGTAAGATATTAGAACAAGAAGAATCAAGTAAGAAAAAATTGGAAG AGCAAAGATTAGAATATGAAAAAAACATTGCTGAAAAGCACAATATAGATGGTAGACTTAAAACTGCTAA GGAACAATATAAAGACCAAAAATCAACACTAAATAAACTATTAGCAGAAAATAAATTTGAAAGTATATAT GCAGTAAAAAGAGCTTTATTAGATACTGATGCAGTAAAAAAATTGATTGAAGAAATACTAGAGCATGAAG AAGAACAAAAGTTATTATCATTTAAGATAAAATCATCAAAAGAAAAATTAAATGGAAGAAGCATAAAGAA GGAGTACTTTGAACAGTTAAAAGATGAAATTTATAATTTGAAAGTAGAAATAGGAAATATATCCAAAGAT ATTGGAGCTAATCAAAATCAGTTAATAACACTAAAAGACTCTTTAGATAAGATAAATGATTTTAATAAAC AACTTAAGGTAGTTGAACACAATGTGGACTTATTGGAAGAATTAGATAAATGTGTGCAAGGAAATAAATT TGTAGAATATGTTTCTACAAACCAATTAAAATATATAGCATTGGAAGCATCAAAACGATTGGATGGAATA ACAAAAGGAAGATATGCACTTGAAATTGACTCAACACTAAACTTTGTAATGAGAGATAACTTTAATGGAG GAGAAAGAAGAAGCATTGATACTCTATCTGGTGGAGAGACATTCTTGACATCTCTATCATTAGCACTTGC TCTTTCATCTCAAATACAATTAAAGGGAAGTGCACCATTAGAATTTTTCTTTTTAGATGAAGGTTTTGGT TCATTAGATAGTGAACTTTTAGAAATTGTTATAGAATCCTTGGAAAGATTGCATAGTAATAATCTAAGTG TGGGTATAATAAGTCATGTCGAGGAACTTAAAAATAGAGTTCCTGTTAAGCTATTAGTTTCATCTAGTGA GGCTGGAATAGGTTCAAAAGTAAAAATTGAATATAGTTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 1112835-1114046	Sequence	CD196_0919 sbcD nuclease subunit D
ORF.. 1114021-1117560	Sequence	CD196_0920 sbcC exonuclease subunit C