Sequence retrieval

Genome: Clostridium difficile CD196
Start position: 2395562
End position: 2396386
Length: 825

DNA sequence
>NC_013315, from 2395562 to 2396386 (825 bp); Clostridium difficile CD196 CTACCCAATTATATCTATAAATTTCTTTATCCATAAATTCCAAAGACGCCATTCATGCCCCCCTACACAC TCTTCATATTCAACATCTACACCCAGAGACTCTGCATATTTTTTAAACATCTGATTATTTTTATATAAAA AATCTCTATCACCACAAAATTGATAAATTTTAGGAATATCTTCATTTCTTTCTTTAAGGTTCTTAAGTAT GTAAAGTAAATCATTCTCACTATTTCTAGCGTCTTCTGGTCTACCAAAATCGTGAAATAAAATGTCTGCA TTGGTTTCTATTTGTTGATTTTCTAACTCATGAATCATATCAGGCGAACCAGAAAAACTTGCAACTGCCG AAAACTCTTTTGGTTTTGAAAGTGCTAATTTTAAAGCTCCATATCCCCCCATAGACATACCAGCTACAAA GTTATCTTCCCTTTTATCTGATAAAGGAAATATAGCTCTAACAAATTTAGGTAATTCCTCGCTTACAAAA GTAAAATATTGGTGTCCATACGCCATATCTGTATAAAAACTAGTTTCTACTCTTGGTATTACTAGGGCTA TATTTTTATTTTTAGCATAATGCTCTACATTAGTATATCTAATCCATGAGCTTTGGTCATCTTCTAAACC ATGAAATAAGTATAATGTTTTAAATTTACCTTCAAAATCATATATCTCTTTACCACTAAAAAGTTCTTTT CCATTTGCTACAGGCAAAATTACAATTATATCAGTTTCTCCTCCAGTATATTTAGATTTAAATTTACATT CTATAAATGCCACAAAAAACACCTCTTCTATAATAATTTTATATCTAAAAAGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2395562-2396386	Sequence	CD196_2068 - esterase (Protein is coded in complementary strand; from 2396386 to 2395562)