Sequence retrieval

Genome: Clostridium difficile CD196
Start position: 3220106
End position: 3221479
Length: 1374

DNA sequence
>NC_013315, from 3220106 to 3221479 (1374 bp); Clostridium difficile CD196 TCACTCCTTAGGCATATATTTTTCAAAATATTCATCACGGATACGTTTAAGTTCACTTTTTGGTAACATA CTAAGAAGCTTCCATCCGATTTCTAATGTCTGCTCAATAGTTCTATCAGTTCTAAATCCTTGTGAAACAT ATTCTTTCTCAAATTCATCTGCAAACTTAGCATACATTAAATCAATCTCAGAAAGTGCAGATTCACCAAG TATAGCCATAAGTTCTTTTGCATCCTTACCTCTTGAATAAGCTGCAAAAAGCTGGTTCATTGTATCTGCA TGGTCTTCACGAGTCTTACCTTTACCAATACCTTTATCTTTTAGACGAGAAAGTGATGGTAAAACATCTA TAGGAGGCTGTATATCTTTTTTATAAAGTTCACGGCTAAGTATAATTTGCCCTTCTGTAATGTATCCTGT AAGGTCAGGAATTGGATGTGTCTTATCATCCTCAGGCATTGTAAGTATTGGAATCATAGTTATAGACCCT TCATGCCCTTTCATTTTTCCTGCTCTTTCATACATAGTAGCAAGGTCAGTATAAAGATATCCTGGATATC CACGACGTCCTGGAACTTCTTTTTTAGCTGCTGAAACTTCACGCAATGCCTCTGCATAGTTTGTAATATC CGTTATTATAACAAGTACATGCATATCTTGCTCAAAAGCAAGGTATTCTGCTGCTGTAAGTGCCATTCTA GGTGTAGATACACGCTCTACAGCTGGGTCATTTGCAAGGTTTACAAACACTACAGAACGGTCGATTGCAC CAGTTGCTTTAAAATCGCTTATAAAAAATTCAGCATCTTCAAAAGTTATACCAACTGCAGCAAATACAAC GGCGAACTTAGAATCAGTTCCACGTACTTTTGCCTGTCTTGCAATCTGAACTGCAAGATTGGCATGTGGC AAACCAGACCCTGAGAATATAGGAAGTTTTTGCCCTCTAACCAATGTATTAAGTCCATCTATAGCAGAGA CTCCAGTTTGTATAAATTCGGCCGGATAATCACGTGCTGCTGGATTTATTGGAGCTCCATTTATATCAAG TCTTTTATCTGGTATAATTTCTGGACCTCCATCAATAGGTCTACCCATTCCACTAAAAACACGGCCTAAT ATATCTGGTGAAACTCCAAAGTCAAGACTCTTTCCTAAAAAACGAACTTTACTTTCAGCAAGGTTTATAC CTGTTGAGCTTTCGAAAAGCTGAACAAGTGCAGTATCTCCATTTACCTCTAGAACTTTGCAACGGCGAAT TTCTCCATTTGAAAGTTCAATTTCTCCAAGTTCATCAAATTTTACACCTTCTACGCCGTTTATTAACATA AGAGGACCGGCTACCTCTTGTATTGACTTATATTCCTTTATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3220106-3221479	Sequence	CD196_2741 ntpB V-type ATP synthase subunit B (Protein is coded in complementary strand; from 3221479 to 3220106)
ORF... 3221476-3223254	Sequence	CD196_2742 ntpA V-type ATP synthase subunit A (Protein is coded in complementary strand; from 3223254 to 3221476)