Sequence retrieval

Genome: Clostridium difficile CD196
Start position: 3622307
End position: 3624187
Length: 1881

DNA sequence
>NC_013315, from 3622307 to 3624187 (1881 bp); Clostridium difficile CD196 TTAGTTTTCGTCTTTTTCGTATATTTCTCTTCTCTTCTTACTCATTGGTAGAGATTGCTCATTCTCTTCT AATACTATTTTTTCTCCTGTAACTTTTTCTATAGCTTCTATGTTATTTAATTTAACATTTGGATTCCATT TTCTTTCAGGAGCTTTAACTTCTACACCTTCTATAGCATTTTTAAGCATAGCCATTATTCTAACAGCATC TTCTGGAGCTAAAGTGTTGTTAGATAATATTTCATTCTCTATACCTTGCTTAGACTTATTGTTGTCTACC ATGTGAGTCATGTATTTATTACCAACAACTAGAGCACCTTGAACAGCTGAGAAACTTACACCAACTACTG GTATACCTCTCATACCTATTTGCTCTATGTGAGATGCGAAATCTATATGGTTGTTTCCGAAACCTTCAGT AGTTACTACAGCTCCATCAACTTCCATAGCTTCAACTAACATACCTAATCTCTTAGATACATAGAATTTC TCAGCATTTACTTGTGGAGAACCAACAAATACAACACCTACTAAATCTATTTCTTCGTCTTCCATAGCTC TTATTACTAATGGCTCTCTCCAGTAATGTCTTGACATTTCTTTTGATGCAGGTCCTATACAAGTTAATGC ATGGATACCACCATCTAATACTTCAAGTGGAGATAATACAACTGGCATGTTTCCTAAGTCAACGTTTGGT TGAGCTCCTAATGTACCAACTGGCTCAACTGGTAATATTAGGTTATCATGCATTGCACCTTGTCCCATTA TTTCTTTTATAACAACAACTTTCTTTTTACCTTCTCTTCTGTATTGCTCTATTACTTCTTCATCAACTAC TAAAGAGTTGTCAACTTTCTTTAATGCTTCTCTTATTTCTTGAGTTACATAGTCAAATGCACGGTGAGCA GCTAAAGGTCCTGGTCTTTCCATGTTAGTTCCTGCTTTTATTGTTACTTGACCTTTGATGAATATTTCAC CTTTGTCAGCAGCACCTGGTCTACCCCACATTATAGTAGTATTTAACTCACCTTCTGAAGAACCAAATTC ACCTATTTGAACTCCATCTTCATCTGTACCAGTTAATACCATTACAGCTCCATCTATAACTCTAGTTATA CCTGAACCTAATTCGCCTTCTTCTTTAGTAGCGATTGGTTGTATATCTAATACAGCTTCACTGTAAGTTT CATATTTATCAGGAGTTATTATTTCTAACTTCATATCTACAACTAACTCTTGAGTTTCATTAGCTTCTTT ACATATTTCTTCTGCGTTTCTGATGTATAAAGTAGTTCCTTCTATTTTAGTTTCATCAGCAAATTCAACT TTATCTATTTTAAAGTGTTTTTTAGTTAAGCTTCTTAAAAGTTTCTTTTCTTCAACTTTTGGAGCTACTT CTGCTGCTGCACTTGCCACTGGAGCAGCAACGTTTGCTACTGGAGCAGCAACTCCTGCTTGACCAGCTAC TGATAAAGGTATCTCTAAGCTTATGTTCTTACCTTCACCTATGTGTATTTTAACTGTTTCACCTGCAACT CCTGTGCTTACTGGAACTACTGCTGCTTCTGATACTGGAGCTGCTTCTTCTACTGTTTCTTCTACTACTT CTTCTTTAGCCTCTCCTGCTTTGTATCCTTCTACGTTATCTGTAGTCATTGGAGAAAGTGCATCTAAAGT TTCTTTTAACTTAGCTCCTAGTACTTGACCTATAGTTAATCCATTTTCTGGTATTGTTAATAATCCAGAA TCCTCTAAGTCTGCAAATATTGCTGGATCTTCTAAGTTTTCTGGCGCTATAATTGTTCCCGCTTCAAATC TACAACAACAAACTGCTGGGTCATTTGCATGGGCTTGAGCTGTTTCTAAAGTTATTGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3622307-3624187	Sequence	CD196_3057 prdA proline reductase subunit proprotein (Protein is coded in complementary strand; from 3624187 to 3622307)