Sequence retrieval

Genome: Clostridium difficile CD196
Start position: 532077
End position: 533321
Length: 1245

DNA sequence
>NC_013315, from 532077 to 533321 (1245 bp); Clostridium difficile CD196 CTATACTTTAAAACAAACAGAATTTTTAATCTCAAGTTTTTTATTTATCTTAGCAACCTTAATCTTAATC TTATCCTTCAAAGGAATACATCCTATATTACTTTCACTTTTTACATCAACACTTCCAACAAATTTACCAG AGTCATTATAATACTTTGCAACACCAACAATATCTCCCTTATGGATAGGATAATTCATATTTTTAAACAC TACCTTAGGATTGATATTTTCGTTATCTGAAACAACACCATACAAATCTTTATCAACCTTAACTGTTATC TTAAAATCATCAATACCATCAACACAAGGAGTCTCTATAATATCACCCTTATGTGCTATCTTTTTAGAAT GGAAATTATCTTTACCATATTTCAATAAAGTAGCGGAAGAAGAAATTCTATTTTTCTTGTTACCATCACC AAGTACAACACCTATCAATCTATGATTTCTTTCGTTTTTAGCATCTTTAGGTACCATCATAGAAAATGCA AGGCAATATCCAGCATTATCTGTATAGCCTGTCTTTATTCCATCAACACCAGGTACTGAAACAAGTAGGG GATTTGTGTTATAATGTGTAAAATCCTTTTTAGTATCATTATATACTTGCATAGTGGTTATTCTTGTAAC TTGATTTTCATAATGGTCATACATATACTTTCCAAGTGTAACTATATCGTGAGCAGTTGACATATTTTCA ATAGGAGGTTCTTTATGTTCTGGGTCAGTATATATAGGAAGCCCATTTGGATTAAAATAATACGTATCAA TCATTCCTATTTCTTCTGCTTTCTTGTTCATCATATTTACAAAATTCTTTTCAGTCTTACCAATATGTTT AGCTATGGCTATAGCAGCATCATTTCCAGATACTAGCATTAGACCCTGCATAAGCTCTCCTAGAGATATT TCATCACCATCTTTAAGTTTACAAGTAGAACCTCCTACTGAAGCTGTAGACTTGTCTATTTTCACCATAT CAGTCTCTTTTACTTGATTCTTATCAACTGCTTCTATTGCCAATAAAAAGGTCATCATTTTACTTAAACT CGCTAATGGCATTTTTTCATCAATGTTTTTTTCAAATAATATTCTTTTAGTTTCCTGGTCTATAAGTAGT GCTGCTCTTGAAGAATTTGGTACTGGTGGAGTATCTGCAAATGCAAATACAGGAGTTATAAATATAAAAA TTAATGCAAGTATTGTTAACTGTTTTATTTTTCCTTTCAAAATCTCGTCCTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 532077-533321	Sequence	CD196_0455 - D-alanyl-D-alanine carboxypeptidase (Protein is coded in complementary strand; from 533321 to 532077)