Sequence retrieval

Genome: Streptomyces scabiei 87.22
Start position: 4598089
End position: 4600935
Length: 2847

DNA sequence
>NC_013929, from 4598089 to 4600935 (2847 bp); Streptomyces scabiei 87.22 CTAGGCGCTCTCCCCGGCCGACGTCGCCTTCTTCGCAGCCGCCTTCTTCGCGGGAGCCGCCTTCTTCGCC GCGGCCGTCTTCGAAGCAGCCGTCTTCGCGGCAGCGGTCTTCTTCGCCGGCGCGGCCTTCTTCGCCGCGG TCTTGGTCGCCGTCTTCTTCGCCGGCGCCTTCTTGGCCGTCTTCTTGGCGGGGCCCTTCGCCCGCTTCTC CGCGAGCAGTTCGTACCCGCGCTCGGGAGTGATCTCCTCCACGCTGTCCCCGGACCGCAGCGTCGCGTTG GTCTCGCCGTCGGTGACGTACGGCCCGAAGCGGCCGTCCTTCACCACGACCGGCTTCGCGCTGACCGGGT CCTCGCCCAGTTCCTTCAGCGGCGGCTTGGCGGCGGCCCGCCCGCGCTGCTTGGGCTGGGCGTAGATCGC CAGCGCCTCTTCGAGGGTGATCGTGAAGAGCTGGTCCTCGGTCTGCAGCGAGCGCGAGTCCGTGCCCTTC TTCAGATAGGGCCCGTACCGGCCGTTCTGCGCGGTGATCTCCTGGCCCTCGGCGTCGGCGCCGACCACGC GCGGCAGCGACATCAGCTTCAGCGCGTCGGCGAGGGTCACCGTGTCCAGCGACATCGACTTGAACAGCGA GGCCGTGCGCGGCTTGACCGCGTTCTTGCCGGTCTTCGGGGTGCCCTCGGGGAGCACCTCGGTGACGTAC GGGCCGTAGCGCCCGTCGCGGGCGATGATCTGATGGCCCGTCGCCGGGTCGGCGCCCAGCTCGAAGTCGC CGCTGGGCTTGGCGAGCAGTTCCTCGGCGAGTTCGACGCTCAGCTCGTCGGGGGCCATGTCCTCGGGCAC GTCGGCCCGCTGATGCCCTTCCGCGTCCTTCTCACCGCGCTCGATGTACGGGCCGTAGCGCCCGACGCGC AGCACGATGCCGTCGCCGACGGGGAACGACGACACCTCGCGGGCGTCGATCGCGCCCAGGTCGGTGACCA GTTCCTTCAGGCCGCCGAGGTGGTCCCCGTCGCCGTTGCCGGCCTCGGCCGCAGTGCCGCCGGAGCCCGT GCCCTCGCCGAAGTAGAACCGCTTCAGCCACGGCACGGCCTGGGCCTCGCCGCGCGCGATGCGGTCGAGG TCGTCCTCCATCTTGGCGGTGAAGTCGTAGTCGACGAGCCGCCCGAAGTGCTTCTCCAGCAGGTTGACCA CGGCGAAGCTCAGGAAGGACGGCACGAGGGCCGTGCCCTTCTTGAAGACATAGCCCCGGTCGAGGATGGT GCCGATGATCGACGCGTACGTCGACGGGCGGCCGATCTCGCGCTCTTCGAGCTCCTTGACCAGCGACGCC TCGGTGTAGCGGGCCGGGGGCTTGGTGGCGTGCCCGTCGACCGTGATCTGCTCGGCGGACAGCGCGTCGC CCTCGCCGACCTGCGGCAGCCTGCGCTCGCGGTCGTCCAGCTCGGCGTTCGGGTCGTCGGCGCCCTCGAC GTAGGCCTTGAGGAAGCCGTGGAACGTGATCGTCTTGCCGGAGGCGCTGAACTCGGCGTCCCGGCCGTCG GAGGCGGTGCCGCCGATCTTGACGGTGACGCTGTTGCCGGTCGCGTCCTTCATCTGGGAGGCGACGGTCC GCTTCCAGATCAGCTCGTAGAGCTTGAACTGGTCGCCGGTCAGACCGGTCTCGGCGGGCGTACGGAAACG ATCACCCGAGGGCCGGATCGCCTCGTGCGCCTCCTGCGCGTTCTTGACCTTCCCGGCGTACGTCCGCGGC TGCGCCGGCAGGTAGTCGGCGCCGTACAGCTGGGTGACCTGGGCACGGGCGGCGGCCACGGCGGTCTCGC TGAGCGTCGTGGAGTCCGTACGCATATAGGTGATGAAGCCGTTCTCGTACAGCTTCTGCGCCACCTGCAT CGTCGCCTTCGCACCGAAGCCCAGCTTGCGCGAGGCCTCCTGCTGGAGCGTCGTCGTGCGGAAGGGGGCG TACGGCGAGCGGCGGTACGGCTTGGACTCGACGGACCGGACCGAGAAGCGGGTGTTCTCCAGGGCGGCGG CCAGCGCACGCGCGTTCGCCTCGTCCAGGTGCAGGACGTTCGCGCCCTTGAGCTGCCCGACCGAGTCGAA GTCGCGGCCCTGCGCGACCCGGCGGCCGTCCACGGACTGCAGACGGGCGACCAGGGACGCCGGGTCGGAG GCGTCACCGGCGCGGCCGGTGGCGAAGGTGCCGGTCAGGTCCCAGTACTCGGCCGAGCGGAACGCGATGC GCTCCCGCTCCCGCTCCACGACGAGCCGGGTCGCGACGGACTGGACGCGGCCCGCCGACAGCCGCGGCAT GACCTTCTTCCACAGGACCGGCGAGACCTCGTAGCCGTAGAGACGGTCGAGGATGCGTCGGGTCTCCTGG GCGTCGACGAGGCGCTGGTTCAGCTCGCGCGGGTTGGCGACGGCGGCCTGGATCGCGGCCTTGGTGATCT CGTGGAAGACCATCCGCTTGACCGGGACCTTGGGCTTGAGCACCTCCTGGAGGTGCCAGGCGATGGCCTC GCCCTCGCGGTCCTCATCGGTGGCGAGGAAGAGTTCGTCGGATTCCTTCAGGAGATCCTTGAGCTTCCTG ACCTGCGCCTTCTTGTCCGCGTTGACCACGTAGACGGGCGCGAAGTCGTGCTCGACGTCCACGCCTAGGC GGCGCACCTCGCCGGTGTACTGCTCGGGCACCTCCGCGGCGCCGTTGGGAAGGTCGCGGATGTGCCCCAC GCTCGCCTCGACGATGTAGCCGGGGCCGAGATAGCCCTTGATCGTCTTCGCCTTGGCAGGCGACTCGACA ATGACGAGTCGGCGGCCGCCCTTCGCGGTCTCGCTGGTCGGGGACAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4598089-4600935	Sequence	SCAB_40781 topA DNA topoisomerase I (Protein is coded in complementary strand; from 4600935 to 4598089)