DNA sequence >NC_014158, from 4159585 to 4159644 (60 bp); Tsukamurella paurometabola DSM 20162 GCCGCGGCGGGATCGAGCGCAGCGGGATCGAGCGCAGCGGGATCGAGCGCGGCGGGATCG Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI