DNA sequence >NC_014158, from 190552 to 190614 (63 bp); Tsukamurella paurometabola DSM 20162 CCGCGTCCCCAGCCGCTCGCCGCGCGTCCCCAGCCGCTCGCCGCGCGTCCCCGGCCGCTCGCC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI