DNA sequence >NC_014158, from 3172122 to 3172196 (75 bp); Tsukamurella paurometabola DSM 20162 GCCCCTAGCGGGAGAGCGACGGTGCGTCCCTCGCGGGAGAGCGACGGTGCGTCCCTCGCGGGAGAGCGAC GGTGC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI