DNA sequence >NC_014158, from 3172129 to 3172203 (75 bp); Tsukamurella paurometabola DSM 20162 GCGGGAGAGCGACGGTGCGTCCCTCGCGGGAGAGCGACGGTGCGTCCCTCGCGGGAGAGCGACGGTGCGT CCCTC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI