Sequence retrieval

Genome: Tsukamurella paurometabola DSM 20162
Start position: 3999217
End position: 3999351
Length: 135

DNA sequence
>NC_014158, from 3999217 to 3999351 (135 bp); Tsukamurella paurometabola DSM 20162 GGCCGCCTTCTTCGCCGGTGCCTTCTTCGCGGCCGTCTTCGTGGCGGTGGTCTTCTTCGCGACCGCCTTC TTCGCGGCGGCCTTCTTCGCCGGCGCCTTCTTGGCGGGCGCCTTCTTGGTGGCCTTCTTCTTCAC
Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF. 3999194-4002100	Sequence	Tpau_3876 - DNA topoisomerase I (Protein is coded in complementary strand; from 4002100 to 3999194)