DNA sequence >NC_014158, from 4159587 to 4159646 (60 bp); Tsukamurella paurometabola DSM 20162 CGCGGCGGGATCGAGCGCAGCGGGATCGAGCGCAGCGGGATCGAGCGCGGCGGGATCGAG Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI