Sequence retrieval

Genome: Isoptericola variabilis 225
Start position: 2558592
End position: 2561561
Length: 2970

DNA sequence
>NC_015588, from 2558592 to 2561561 (2970 bp); Isoptericola variabilis 225 TCACTCGTTGAGGCCGTGGCACACCTTGTACTTCTTGCCCGAGCCGCACGGGCACTGGGCGTTGCGCGGC GTGCCCGGGAACGTCCGGCCGTCGGCCTCGGCGGCCGCGGCCTCGCCGTGCAGGGCACGGCGCGTCTTGC GCGAGTCGCCGGCGACGGAGGTGGAACCGTCCTCGCTGGGTGCTGTGTACTGCAGGTTCTGCGGGCGCTG CGGGGCGTCGAGGCCCTTGGCGACGAGCACGGGCTCGTCGCTGCCACGGAACGACGCCGACACCTCCGGC TTCGCGGGCTCCGCGGGCGGAGCCGGCTGAGCCGCCGGAGCCGGCTGCGCGGCCGGCTCGCCCGAGGGCT GGGCCGGAGCCACGGGGGCGGGCGCACCGCCCGACGCCGCGATCGCCTGCGCGGCGGCCGACTGGGCGGC CGCGGCGGCGCCGGCGGTGGTGACCGACGGACCCGTGGCCGTACCGGGCTGCTGGACCTGCACCTCGAGG TTGAACAGGTACCCGACCGACTCCTCCTTGATGGCCTCGGTCATGGCCTGGAAGAGCTGGTAGCCCTCGC GCTGGTACTCCACGAGCGGGTCGCGCTGGGCCATGGCCCGCAGGCCGATGCCCTCCTTGAGGTAGTCCAT CTCGTAGAGGTGCTCGCGCCACTTGCGGTCGAGGACCGAGAGGACGACCCGGCGCTCGAGCTGGCGCATC GCGGCGGAACCGAGCTGCTCCTCGCGCGCCTCGTACGCGACACGGGCGTCGGAGAGCAGCTCGTCGAGCA CCATCTCGCGCGTGAGGCGCCCGGCGCCGCCGGCCTCCTCGACCACGTCCTCGGGCGTGATCGACACCGG GTACACGGCCCGCAGCGCGGTCCACAGGGAGTCGAGGTCCCACTCCTCCGGCGTGCCGGACGACGTGGCG CCGTCGACGTACGCCGTGACCACGTCGGTGAGGAAGTGCTGCACCTGCTCGTGCAGGTCCTCGCCCTGGA GCACCCGCCGACGCTCGGCGTAGATGACCGAGCGCTGGCGCGACAGGACGTCGTCGTACTTGAGGACGTT CTTGCGGATCTCGAAGTTGCGGGCCTCGACCTGCGACTGCGCGCTCTGGATGCCGCGCGTGACCATCTTC GACTCGAGCGGCACGTCGTCCGGGAAGCCCGCCCGGTTCATCATCGACGACGCCAGGCCCGAGTTGAACA GGCGCATGAGGTCGTCCTCCATCGACAGGTAGAACCGGGACTCGCCCGGGTCACCCTGGCGGCCGGAGCG ACCGCGCAGCTGGTTGTCGATGCGGCGCGACTCGTGCCGCTCGGTGCCCAGCACGTACAGGCCGCCGAGG GCGACGACCTCGTCGTGCTCGGCCGCGACGGCCGCCTTCGCCTTCTCGAGGACCTCGGGCCAGACGGCCT CGTACTCCTCCGGGTCCTCGGCGGCGTCGAGGCCCCGCTCGGCCATCTCCTGGACGGCGAGGAACTCGGC GTTGCCGCCGAGCATGATGTCGGTACCGCGGCCGGCCATGTTCGTGGCGACCGTGACGGCACCCTTGCGG CCCGCCTGCGCGACGATCGCGGCCTCGCGCTCGTGCTCCTTGGCGTTGAGGACGTTGTGCGGCACGCCGG CACGCTTGAGCAGCTGCGACAGCCGCTCCGACTTCTCGACGCTCGTCGTGCCGACGAGCACCGGCTGGCC CTTGGCGTGGCGCTCGACGATGTCCTCGACGACGGCGGCGAACTTGCCGGCCTCGTTCTTGTAGACCAGG TCGGGCTGGTCGATGCGGATCATCGGGCGGTTGGTCGGGATCGGCACGACCCCGAGCTTGTAGGTGCTCT GGAACTCGGCCGCCTCGGTCTCGGCCGTACCGGTCATGCCCGCGAGCTTGTCGTACAGGCGGAAGTAGTT CTGGAGGGTGATCGTGGCGAGCGTCTGGTTCTCCGCCTTGATCTGCACGCCCTCCTTGGCCTCGATGGCC TGGTGCATGCCCTCGTTGTAGCGGCGGCCGGCGAGGATACGGCCGGTGTGCTCGTCGACGATCATGACCT CGCCGTTGAGCACGACGTAGTCCTTGTCGCGCTTGAAGAGCTCCTTGGCCTTGATCGCGTTGTTGAGGAA GCCGATGAGTGGCGTGTTGAGCGACTCGTACAGGTTGTCGATGCCGAGGTAGTCCTCGATCTTCTCGATG CCCGGCTCGAGCACGCCGACGGTGCGCTTCTTCTCGTCGACCTCGTAGTCCTTGCCGGCCTCGAGACGGC GCACGACCTTGGCGAACTCGACGTACCAGCGGTTGGCGTCGCCCGACGCCGGGCCCGAGATGATGAGCGG GGTCCGCGCCTCGTCGATGAGGATCGAGTCGACCTCGTCGACGATCGCGAAGTGGTGGCCGCGCTGCACC AGGTCGTCGGGCGACCAGGCCATGTTGTCGCGCAGGTAGTCGAAGCCGAACTCGTTGTTCGTGCCGTAGG TGATGTCGGCCGCGTACTGCTCGCGGCGCTCCGCCGGGCTCTGGCCCGACAGGATGATGCCCGTCGTCAT GCCGAGGAACCGGTAGACGCGGCCCATGAGCTCGCCCTGGTACTTGGCGAGGAAGTCGTTGGTCGTGACG ACGTGCACGCCCTTGCCCTCGAGCGCGTTGAGGTACGCGGCCGTCGTGGCGACGAGCGTCTTGCCCTCAC CGGTCTTCATCTCGGCGATGTTGCCGAGGTGCAGCGCCGCCCCGCCCATGAGCTGGACGTCGAAGTGGCG CTGACCGAGCGTGCGGCGTGCCGCCTCGCGCACGGCCGCGAACGCCTCGGGCAGGAGGTCGTCGAGCGTG GCGCCGTGTGCCAGCCGCTCCTTGAAGCGGTCCGTCTCCTCCCGCAGCTCCTCGTCGCTGAGCTCGACGA AGCTCGGCTCGAGCCGGTTGACCTGCTCGGCCAGACCGGTCAGCTTCTTGAGGATCCGGCCCTCGCCGAA GCGGAGGACCTTCTCGAGGATCGCAGGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2558592-2561561	Sequence	Isova_2350 - protein translocase subunit secA (Protein is coded in complementary strand; from 2561561 to 2558592)