Sequence retrieval

Genome: Azospirillum lipoferum 4B
Start position: 1112951
End position: 1113160
Length: 210

DNA sequence
>NC_016622, from 1112951 to 1113160 (210 bp); Azospirillum lipoferum 4B TCAGGTCCGCGTGATGCCGCCAGTCCGATCCGGTCCGATGGGCCTTGTTCGGCCTCGTTCGAGATTATGC CGCCGAGCGCCGAGCGCCGAGCGCCGAGCGCCGAGTGCCGAGTGCGGGCGGGCGGCGTTGTAACAATCGA TCCAACGGCGGCGCCCCTCCATCGACATCCGAATGCCGGCCGACAGGCGCGGACGCTCCGGCTCGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1112951-1113160	Sequence	AZOLI_1167 - hypothetical protein (Protein is coded in complementary strand; from 1113160 to 1112951)