Sequence retrieval

Genome: Azospirillum lipoferum 4B
Start position: 1472884
End position: 1474188
Length: 1305

DNA sequence
>NC_016622, from 1472884 to 1474188 (1305 bp); Azospirillum lipoferum 4B TCAGAACTGCTCGATCCGGATCATCCGCGGCGGCTCCAGCACGTTGTCGCTGGCCGCGATGGTCGCAAGC GCGCGCTGCATGGCCGCCTCGTCGGTATCATGGGTGGTGAGGACAACCGGAACGCCTTCGCCCGGCGAGC GGCCGCGCTGCAGGAACTGCTCCATGGAGACGTTCTGGTCGCGCATCGCCGCTGCGATATCCGCTATCAC ACCGGGGCGGTCCACCACCATCAGGCGGACGTAGTACGACCCGCGGCGCGATTCCATCGGCGAGGGGGTT GCCGCGCCCAGCCGATCGGCCGGAACGCCGAAGGTGGGCGTAGACCGGCCGCGCGCGATGTCGATCAGGT CGGCGACCACCGCCGACGCGGTCGGGCCGGAGCCGGCGCCGCGGCCGACGAACAGCACCCGGTCGGCGAA ATCGGCCTGGGCCACCACGGCGTTGAACACGCCGTCCACCGACGCGATGGGGGCGGTCTTCGGCACCATG CAGGGATGCACCCGCTGCTCGACACCCGCATCGGTGCGGCGTGCGATGCCCAGCAGCTTGATGCGGAAGC CCAGCTGGTCGGCATAGTCGAAATCGACCGCCGAGACCTGCCGGATGCCTTCGATGTAGACGGAGGGGAA GTCGACCGGCGTTCCGAAGGCGACCGAGGCGAGGATCGCCAGCTTGTGCGCGGCATCGACACCATCGATG TCGAAGCTGGGATCTGCCTCGGCATAGCCGAGGGCCTGGGCATCGGCCAGCACATCGGCGAAGTCGCGGC CGGTGGTCCGCATCTCCGTCAGGATGTAGTTGCAGGTGCCGTTCAGGATGCCGTGGATTTCCGATACGGC ATTGGCTGCCAGACCTTCGCGCAGCCCCTTGATGATCGGGATGCCGCCGGCCACCGCCGCCTCGAAGGCG ACGGTCAGGCCGCGGGACTCGGCCTTCCGGGCGATCTCGGTGCCATGAACCGCCAGCAGGGCCTTGTTGG CGGTGACCACATGCTTGCCGGTCTCGATGGCGGTGGCGACGGTGTCGCGCGCCGGACCTTCGGAGCCGCC GATCAGTTCCACCACCAGATCGGCGCCGGACTGGGCCGCCATGGCGACGGGATCGTCGAACCATTGGACC TTCGACAGATCGACGCCACGGTCCTTGCCCTTGGAGCGGGCGCTGACCGCGACCACCTCGATCCGGCGGC CGCAGCGCTGTTCGATCAGCGAGGCTTGGGCGTCCAGCAGCTTCAGGACGCCGGCGCCGACCGTACCGAG GCCGGCGACGGCGATTTTCAGGGGGGCTTGCTGGGCGTTCGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1472884-1474188	Sequence	AZOLI_1546 hom homoserine dehydrogenase (HDH) (Protein is coded in complementary strand; from 1474188 to 1472884)
ORF... 1474188-1475462	Sequence	AZOLI_1547 dapL LL-diaminopimelate aminotransferase (Protein is coded in complementary strand; from 1475462 to 1474188)