Sequence retrieval

Genome: Azospirillum lipoferum 4B
Start position: 1491805
End position: 1492473
Length: 669

DNA sequence
>NC_016622, from 1491805 to 1492473 (669 bp); Azospirillum lipoferum 4B TCAGAACTTCTGCGACCGCCGGATCATCCGGTCGATCTCCTGCTGAACGAGCCGCTCGACCACCGTCGGC AGATTCTCGTCCAGCCACTCCTTCAACAGCGGCTTCAGAAGTTCGCGCACCACCTCCTCCACCGTGCGGA TGCCGATGGGCATCGGGCCGATGGACAGGTCGTCGCCCAGCTCGCGCGCCAGATGGGTCAGGTGATGCGA GGCGTCTTCCGCCGTCCGGCGGGAGATCAGCCCGTCATCCACCTCGACCGGCCGGCGGCGCGGACGCGGC GGGGGGGGCGGCTCGTCATCCTCGAACTCGTCGAAGGCCGGCATCGGCCGGCGCGCCGGGCGCGGCGGCG GCATCGGCTCGGGCTCCGGCTCCTCCCAACGGGGCGGAGGCGGCGGCGGGGGCGGAGGAGGCTCCGGGAA GGCCGGTTCGTCGGGCCAGGGATCGGGGTCCGGCTGACCGAAGTCCGGGCGTTCGTCCGGCTCGTCCTCC ACCATCTGGGTCAGTTCCAGAACGTCGTCGTCCTCCTCCTCCACCATCGGGGGTGGCGGGGGCGGCGGCG GCGGAGGGGGCGGCGGAGGTGGAGGCGGCGAAGGCGCCTGGGTTTCCGACTTGGCCGGCTCGCCGTCCTC GGAAATGATCCGCCGGATGGAGGCGAGGATCTCCTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1491805-1492473	Sequence	AZOLI_1560 - hypothetical protein (Protein is coded in complementary strand; from 1492473 to 1491805)