DNA sequence >NC_016940, from 3529318 to 3529485 (168 bp); Saprospira grandis str. Lewin GAGGTGAACCAGTTCGGTCTGGAGGTGAACCAGTTCGGTCTGGAGGTGAACCAGTTCGGTCTGGAGGTGA ACCAGTTCGGTCTGGAGGTGAACCAGTTCGGTCTGGAGGTGAACCAGTTCGGTCTGGAGGTGAACCAGTT CGGTCTGGAGGTGAACCAGTTCGGTCTG Translate to protein Restriction digest BLAST Design primers with primer3