Sequence retrieval

Genome: Saprospira grandis str. Lewin
Start position: 362170
End position: 362358
Length: 189

DNA sequence
>NC_016940, from 362170 to 362358 (189 bp); Saprospira grandis str. Lewin GGCATAACTAGTTTACCGCCAAACCTAACTAGTTTACCTCCAGACCTAACTAGTTTACTTCCAGACATAA CTAGTTTACCGCCTGACCTAACTAGTTTACTTCCAGACATAACTAGTTTACTTCCAGACCTAACTAGTTT ACCTCCTGACCTAACTAGTTTACCTCCAGACATAACTAGTTTACCTCCA
Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF. 362158-362490	Sequence	SGRA_0380 - pseudouridine synthase, Rsu (Protein is coded in complementary strand; from 362490 to 362158)