DNA sequence >NC_016940, from 3729365 to 3729469 (105 bp); Saprospira grandis str. Lewin GGTCTGGAGGTGAAGCAGTTCGGTCTGGAGGTGAAGCAGTTCGGTCTGGAGGTGAAGCAGTTCGGTCTGG AGGTGAAGCAGTTCGGTCTGGAGGTGAAGCAGTTC Translate to protein Restriction digest BLAST Design primers with primer3