Sequence retrieval

Genome: Clostridium acetobutylicum EA 2018
Start position: 725477
End position: 725698
Length: 222

DNA sequence
>NC_017295, from 725476 to 725697 (222 bp); Clostridium acetobutylicum EA 2018 TTGTCATCCAAAATTAACTCCTCCTCACATAGTTTTATTGATATATGGAACATTTATATTATGCCTAATA TAAATAATTTAATTCATATATTGAATAAATTTTACTCTTTCTTTTTATATTATTTAATATTTTATTTTAT TAAACTCTATTTTCAATTTAATAAAATAATGGTAAAAAAACAAAAAATTAAAAGTTACTATTGTAAACAT GCGTTTATATAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 725278-725529	Sequence	CEA_G0636 - hypothetical protein (Protein is coded in complementary strand; from 725529 to 725278)
ORF.. 725477-725698	Sequence	CEA_G0635 - hypothetical protein