Sequence retrieval

Genome: Helicobacter pylori F30
Start position: 462655
End position: 464472
Length: 1818

DNA sequence
>NC_017365, from 462654 to 464471 (1818 bp); Helicobacter pylori F30 TTATTTTTTCAAGCTAATAGCTTCTTTAATCATGTCATCGCCCGCGCCAAAGGCTCTAGCGTTCATAGAA TAGCCTCTTTGCATTAAAATGAGATTGGTTAGGGCGTTCCCGGCATTCACATTGCTCGTTTCTAAATATT TATGCCTGATTTTCCCAAACTTGAGTTTGCCCTCTTCGTCCCACCCTAAAATGGGGTTACCGCTTAGGGG TCTATTTTCGCCATTAATGGTGCCTTCTTGCATTTCATAGAGGTTACCGCCGATTTTTCTCAAGCCTTGA TCGTTAGTGAAAGCTAAAATACCGATGCGAGCGACCGGCTCTACCACCCCATTACTAAAGGCCAGAGAAA TCACGCCATTTTCTTCAATGCGCATGTCTCTAAAAATGCCCCTAGGCTTGCCGTCTTGGCTCGCTTCTAA TAGTTTAGAGTCTTGGTAAGACAAGTCGCTAGAATCATGGTTTTCGCTTTTATCTAAAGAGTAGGTGAGC TTATTGCCTTTAAAATCCAAAGTCATGGGGGCGGATTTCATCTTGTGCGTGGCTGAATCAAAGCCGACAA TTTCCCAAGTAGGATCATTGATAGGGGTTTTGTTTTTGGGATCTACGATGTAGCTTTTCACTTCCCAAGT TTGATTTTTCCTTTTACTCGTGGGGCTTGATGTGGGATCGTTGGAATTGGTCATGTAATATTCGCTTTGG AGCAAGTATTTATCCCCGGCTTTATCATAAATATCCACTTCTGTGCTGTAATAAGGGATTTTAATCGCTA CTGAATCCCTGCTAATGCCCTTTTTTAGTTCCATTCCGTTCGCATTCAAGCCCAATTTATCCGCAATGCC CCCGCTCAAGCTGAATGTGATAGGCGTTTGGCTAGGATTAGCGATCTCTAATAAAAGGGGTGGGCTTTTG GCGTCTTTTTCGCTTTTGATGAGATTCAAGTCTAGCCCGGTTTTTTCTTTAAAGAGTTTTTGCAAATCGC CTAAAGTTTTGAATTGGTTTTCGCCTTTTTCAGCGTCCCCATAAGTGAAAACCAAATCCTCTTTTTTGCC ATCTTCTTTTTGGATACTGATGTTTAATTTACGATTGGTGATCGCATCTATTGGCTCGTTATCGTCATTC GCTAAAGCGTTAATGTCCTGGTTTAAAAAACGCTCTTTAATGATCTCGCCCTTGTCGTTTAAGAAAAAAT CTTGCACGCCTTTTAAATGGTCTTTAGGGTTTAGATTCACGCTAATACCCACTTTCGTGCTAAGCACGGG CTGGTAAGTCAAATCTTGGGGGATTTGTAAGGGCGAAAGGGTGTTGCCATGCAATTTTTCAATGTCTTCA TCTCTAGCGGTGGAATTGAGCGTGCCGTCTTTGATTTTTTTCAAATCAATGCCATAAACATAATAGCCCT CAGGGGTTACTAAATAGCCATCAGCGTCCCTGGCAAAATTACCGGCGCGCGTGAGGAAATTATCCTGTTT CTTGCTAAAGCCGTCTTTATTAATGGTTATTTCCCCATTTTTATTGGGCCCTATCACAAACCAGCCCTTG CCTTGATACGCCATGTGGAATTCCCCCTCGCTTGGCATGTATTCCCCATCTTTATTAGAAAGGACATTGC CTGAGCCTGTAACGCCGTAATTTCGGTCATTAGCCACAACGGATTTGGCGTTCAAAGCGTCTAAATGCGA AGAAAATAGGGTCTTAAACTCCGGATCATTGGAGCGATAGCCTAAAGTATTGACATTGGCGATATTGTTT GAAAGGCTGTCAATACCAAACTGGTGGGTCTTAATCCCGCTATAAGCGTTTAATAAGGTGTCGTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 462655-464472	Sequence	HPF30_0431 flgE flagellar hook protein FlgE (Protein is coded in complementary strand; from 464472 to 462655)
ORF... 464469-465458	Sequence	HPF30_0432 flgD flagellar basal body rod modification protein (Protein is coded in complementary strand; from 465458 to 464469)