Sequence retrieval

Genome: Helicobacter pylori 83
Start position: 130247
End position: 132271
Length: 2025

DNA sequence
>NC_017375, from 130246 to 132270 (2025 bp); Helicobacter pylori 83 TCAAATGCGCAAATAGTTTCTTAAAATGCGCCCGTATTGCGGACTTCTCAATTTTTTAATCGCAGAGCTT TCAATCTGGCGCACCCTTTCTCTAGTAACATTCAATTCCTTGCCAATTTCTTCTAAAGTGCGATCACTTT CATCGTCTAAAAGCCCAAAACGCATGCGAATCACCGCTTTTTCTCGCTCATTCAACTGATCCAAAACGCT TTCAATTTGCGCTTTTAAATCTTCGCGCATGATGTGATCAATGGAGCTAACGATATTTTTATCTTCCACG AAATCCCCAAATTTGCCGTCATCATCATTGCCGACTGGAGTTTCCAAACTGATAGGCTCTTTGGTAACCT TAATCACATTCTTCACTTTATCTAACGAAAGCCCCACTTCTTCAGCCACCACTTCCAAATCAGGCTCTTT GCCGTTTTCTTGAATGTGTTTGCGCATGACTTTATTGATGCGATTAATGGTGTCAATCATGTGAATGGGG ATGCGAATAGTGCGGGCTTGATCGGCTATGGCTCTGCTGATAGCTTGTTTGATCCACCAGGTCGCATAGG TAGAAAACTTGAAGCCCTTTTCATGCTCAAACTTATCCACCGCTTTCATCAAGCCAATATTACCCTCTTG AATCAAATCCAAGAATGGCAAGCCTCTGCTCGTGAATCGCTTAGCGATGCTCACCACCAACCTTAAATTG GATTTAGCCATTTTGTTTTTAGCGCGATCGGAAATCAATTTCCCTCTTTTGATTTGCTCTAAAATTTCTT TTAGCTTGTTGGGGTCTAAATCAAAACCCTCTTCGCTCGCTTCTTTAGTCAAAAAAAGCTTTTTAAGATC CATATACACGCTCACCATAGTCGCTTCTGGCACTTGAGCGATAATGTTCTCTTTAGTCATGTTAGTGATA TTGGCAAGGATTTTTTTATGGTTAGCGATTAGGGTGTCATTGAATAAGGGCAGTTTGTATTCCAAGCGTT TCAACTCTTTTTCAAACCCATCGCCGCTTTTTAAAGTGGTTTCCATCGTTTTGACTAATTCATTAATCAG TTTGCTAGTAGGCTCTAAGTCATAGAGTCTGTCTTTGAGCGTTTGGCGTTTGTAAGCTAGGGTCAATAAA CGCACTAGCTCATCTTCTTTTTCATCTACAGGGGCTTCAAGGGCTTTGAGCCATTCTTTTTTAGCCTTAT CTAGGGCTTTAAAGCTTTCTTGAACCTTTTCTACACGCTTCCTGTCTTTTTCAGAAACGACTTTTTTCCT TTCTTCGTTTTCTTCATCTTCTTCGCTGTCATCATCTTTTTTAGAATCGCTCACGCTATTTTCATCATCA TCAAAGCTCCTAAAAAGCTCTTTAACCCTTCTTTCACGATTGATTAAAGCGTCTTTATACGCATAGATAA AATCAATCAAATACGGCACCGAGCAAATCGCGTCTAAAATAATGTCTTCGCCCAAGCGGATTTGCTTGCT CAATTCAATCTCTTCATCTTTGCTTAAAAGCTTTATATCCCCCATTTCGCGCAAATACATGCGCACCGGG CTATCGCTCCTGCTCCACTCTAAAAAATCCTTTTCTTTCAAAAAGTCATAGCCATCTTCTAATTCTTCAT CTAAAACTTTTTGCTTTTCTTCAGTTTTTTTAATCTTGTCAATCGCATTGAGTTTTTTAGCGTATTCTGA AGAACTGACTAATTTCTTTTGGTATTTTTGGCACAATTCTTTGATTTTTTTGACTTGGGCTAGAGTGGGG ACTTTCATGCTGATTTGAATGATAGACTCATAAGAAACGCAATCGCTTAAGGAATTAGCGAACAATTCTT CTAACGCTTCATTAAAACTAAGCTTTTTAACAGGAACAGGTTCTTTCGCTTTAATTTCTTTGACTTTGCT TTCTTTGGTTTTAGTTTCTTTAATTTTGCTCTCTTTAATTTTGTTTTCTTTGGCTTTATTTTCTTGTGTG GCTTCTGTTTTGTTTTTTTTTGGGGCTTTTTCTTCGTTAGCTTTCTTTTTCATTGGACACTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 130247-132271	Sequence	HMPREF0462_0141 rpoD DNA-directed RNA polymerase sigma subunit RpoD (Protein is coded in complementary strand; from 132271 to 130247)