Sequence retrieval

Genome: Helicobacter pylori 83
Start position: 474361
End position: 474900
Length: 540

DNA sequence
>NC_017375, from 474360 to 474899 (540 bp); Helicobacter pylori 83 TTAAGATTTATAATATTTAATGGTGTAATCTATCCCCGAATAAAGGGTTAAAAAAACCGCTATAGCCACA AGTGCATAAGAGTAAGTTACATCAGCCAATAAAGCGCCAATCGCCACGACTTGAGAAACGGTTTTATACT TGCCTAACGCATTGACAGGAATATCCTTTTTTTCATTAGCGGCTAAGACTCTAAGCCCTGAAATAAAAAA TTCACGCCCTAAAATCACAAACGGGATCCATGCATTCACACGATCCAAATGGACTAACCCTAAAAATGCG CTCAAAATAAGGATTTTATCCGCTAAAGGATCAAAGATTTCCCCAAAGCGCGATTTGGCTTTATAGCTTC TAGCGATGTAGCCGTCCAATAAATCCGTGAGCGCGGCAAACAAAAAAACCAATGAAGAGATCATGTTGAT GTGAAAGGGGGTTAAAAAACTAAAATAAGTGCGCGTGTTTAACAATAAAAATAATAAAAACAAGGATAAG ACAATGCGTAAAATCGTTAAAAAATTAGGCAGGAGTTTTAAAATTTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 474361-474900	Sequence	HMPREF0462_0491 pgsA CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (Protein is coded in complementary strand; from 474900 to 474361)
ORF... 474897-475505	Sequence	HMPREF0462_0492 - hypothetical protein (Protein is coded in complementary strand; from 475505 to 474897)