Sequence retrieval

Genome: Staphylococcus pseudintermedius ED99
Start position: 454150
End position: 454410
Length: 261

DNA sequence
>NC_017568, from 454149 to 454409 (261 bp); Staphylococcus pseudintermedius ED99 CTATCTGTCCAAATGCAAATTCAAATCAAAAATATTCCAAAGTCTAATTTGAGGTTCAACACTCATAAAC TTCGAAAGCTTTTCAGCGCTATTTTTATTAAGGTTTCCAACAATTGTTAGAGCATCTAAACTTAAACTTT GAATAAGTGGGTGCACAGAATGTACTCCTAACCGGCTATTAGAGTATCCGGTTGTGGCGTAATTTATAAC CTCTGTCGATGTGCCTTGTTTTGAATGATTTTCTTGAATATTTCCAGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 454150-454410	Sequence	SPSE_0384 - replication initiation protein, truncated (Protein is coded in complementary strand; from 454410 to 454150)