DNA sequence >NC_018289, from 4378996 to 4379095 (100 bp); Mycobacterium smegmatis str. MC2 155 CTAGAAACTCAGCCGCTACATCCACTTGGCGGCTCAGCCGCTACATCCACTTGGCGGCTCAGCCGCTACA TCCACTTGGCGGCTCAGCCGCTACATCCAC Translate to protein Restriction digest BLAST Design primers with primer3