DNA sequence >NC_018289, from 5644225 to 5644329 (105 bp); Mycobacterium smegmatis str. MC2 155 GAGGAACTGGAGACCACCAACGAGGAACTCCAGTCCACCAACGAAGAACTCGAGACCATGAACGAGGAGT TGCAGTCCACCAACGACGAACTGCACACCATCAAC Translate to protein Restriction digest BLAST Design primers with primer3