DNA sequence >NC_018289, from 5439904 to 5440038 (135 bp); Mycobacterium smegmatis str. MC2 155 CCGGGACGTTCACATCCGGCACGTTCACGCCGGGCACATTCACATCCGGCACGTTCACGCCGGGCACATT CACATCCGGCACGTTCACGCCGGGCACATTCACATCCGGCACGTTCACGCCGGGCACATTCACAT Translate to protein Restriction digest BLAST Design primers with primer3