Sequence retrieval

Genome: Francisella tularensis subsp. holarctica F92
Start position: 665136
End position: 666017
Length: 882

DNA sequence
>NC_019537, from 665135 to 666016 (882 bp); Francisella tularensis subsp. holarctica F92 CTAAACAAAAGTTCGCTTTGCTTGAATGCGTTGCATCCATAGGATAGGTAAAACTAGTATAACTATTAAA ACTACGGATATCGTAGCTGCCATACCCCAATTATTAGATGTGAAAAACTCTTCCCAAATAACATTACCAA TCATTAATGAATCCATACCACCCATAATTTGTGGCACAACTACTTCACCAATAGCTGGGATAAATATTAA CAAGCTGCCAGCAATTAGCCCAGGCATTGATAATGGTAGTGTTATCTTAAAGAAAGAATTAATCGGCCTA GAGCCTAAATCCTCAGCAGCATCATAGATACTAGGATCGATTTTAATTAGAGTGCTATACAGTGGTAACA CTAGAAATGGTAAGTAACAATAAACCATACCTAAATACATCGAAAAATCATTGTAAAGTAAAGCCATGCT TGGTAAGCCTAAATCCATTAGAAATTGATTTAAGGTATGGTTACCTAGTAATGTCACCCACGCATATGTT CTAAGTAGAAATGAGGTCCAGTATGGAATGATAATTAGAACTAAAAAGAAAATCTGGGTGTTCTTTTTGG CTTTTGATAATGCTAATGCCATCGGATAACCGATTAAAATACACAATACTGTAGTTATAAAAGCAACTTT AAAAGATTTAAGTAATGATATGAATATTAGATTATAATGAATCAGCTCAATATAATTACTTAGATATAGC GTAAAGTGTAGTGCTGATTGTTTATAAGTCATCAACGAAGTAACAGGCGGAGTACCATCAGCGGGTAAAG TAAAGCTAATACCTACGACAAATAAAAATGGTATAAGCAGAAAAACTACAAACCATATAAAAGGAATTAG ATAAACTAAAGTATGTCTAAAGCTTTTTTCTAAGTTTTGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 665136-666017	Sequence	F92_03715 - polyamine ABC transporter permease (Protein is coded in complementary strand; from 666017 to 665136)
ORF... 666017-667138	Sequence	F92_03720 - Putrescine transport ATP-binding protein potG (Protein is coded in complementary strand; from 667138 to 666017)