Sequence retrieval

Genome: Francisella tularensis subsp. holarctica F92
Start position: 96530
End position: 97924
Length: 1395

DNA sequence
>NC_019537, from 96529 to 97923 (1395 bp); Francisella tularensis subsp. holarctica F92 TTATATTTCTTCTTTTGAGATAGTTTGTCCTGAAGTATTTGGAGTTGGCTTTTGATTGCGCTCATTTTTT TCCTTTGCTTGTTGTTTTCTTTCGTTAGAAATAGTACGTCTTAATAGGGTATCATATATTGGTTTTTGAT GATGAATATAGGTCAGCATAGATGCACTAAAGCAAGTTATCATCAAAGGAAGTAAAAGATGGAAATTCCA AGTCATCTCCATTACTAGTACAATACCCGTTAATGGTGCACCTACAGTTGCTGTAAATAGTGCACTCATT CCAGCAACTGCAAAAACTCCAGGTTCAATATTATACTGAGGGAAGAGTTGCTCCACTGATAAACCATAAG CTAGTCCAAAAACAGTACCAAGCGCAATCATTGGTGCGAATATCCCACCAGTAACGCCGGTGCCATATGA GAAAATAACTCCAGCAAAACGAAGTACAAAAAGCACTAAAAGCATCTTGATTGATAAGTTATAATCAAGA GTATTTGCTATGACAATATAGCCACCGCCAACAGCATTTGGAGATAGAACAACACCAATACCAAAAATTA TGCAAACAATTATAACTAAAGTCCAATACCTCTTCTTGGATCCTTCTGAGAAAAAGTTTGCCACTTTGAT TAAGGATTTGTTAAATAGTAAACCAAAATAACCAAATATAATCCCTAATACCATAAATAACCAAAGAGTA TTTTGTGGTACTGAGCTGAAAGTTTCTACGCGTATTGCTGGAGGATTACCCATAATAGCTCTAGAGATAA CTGTACTCATGATACATGCTACTAGCACACACTTTATTGCCGAAACACTAAATCTAAACTTTCTATTCAT CTCTTCAATAACAAAGATAATCCCAGAAAGTGGGGTATTAAAAGCAGCTGCTAGTCCAGCTCCAGCCCCA GCAGAGATAACCGCATTAGCATATTTTGTAGTAAGTTTAAATTTATCAACAAAAAACTGCGCTAATGCAG CAGCCATATGAATTGATGGTCCCTCTTTACCTAAACTTAAACCTGAGCCTAACGAAAAAAGTCCACTTAT AAATTTCACGGGCATAACTCTTTTACGTATTTTGCGGCAGCCTTTTAAAGCACCCTCAACCTCTTGGATA CCGCTACCACCAGCCTCTTTCGCAAATTTTTTAACAATAAAAATCGAAATTAATACCATCACAATACTTA ATGATATTGACATAGCGATTTCGATAAAAAGATTACCACCACTGAAAGAAAAAAGAGCCAGCTTAATTTT AAAAATAAAGTCTAGGAGTAGCTGAAATGCTGTTGCTAGAAGACCAATCATTGTGCCTATAACAGCACCA GATAGTACTAGCACCCAAATATGTCGATTGTTTTTATAATATGTATCTAATACCTTATTACTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 95089-96594	Sequence	F92_00555 - metallocarboxypeptidase (Protein is coded in complementary strand; from 96594 to 95089)
ORF.. 96530-97924	Sequence	F92_00560 - chloride channel protein (Protein is coded in complementary strand; from 97924 to 96530)