Sequence retrieval

Genome: Mycoplasma cynos C142
Start position: 519214
End position: 520242
Length: 1029

DNA sequence
>NC_019949, from 519213 to 520241 (1029 bp); Mycoplasma cynos C142 TTAGACAAGTTGTTTTAAAATTTTCTTATTTTTCATCTTACTTATCGGAAACAGCATTAATGTTACTTCT TCATTATTTCTTGAAAAATAAGGGTTAGTAAGTAAATAATTTTCTGCTTTATAAAATGCCTCTAATTTTT CTAAAAGTTCTAAGTGTTTAGATAATAATAATTTGCTTCTTTTTTCATGAATTTCTTCTTTTGTTTTATA AATATTTTTATACGTAAAACTTGTTTCTTTCAAAAAGCCGGTCATTAAGAGTTTCAAAAAATAATATTTA GCATAAGTGATTTCCTCAAATAACTTGCGATTCATATTAAGTAAATATTTCTCAAGTTCATCAAATATAT TAAGGACTCTTTTAGAATTTAATCACATATCAGCAGGAAAATATTCACGATAAATTTTAAAATCTAAATA AGTATAACTTTTTGCTTCAAACAATAAAATATAATTCAATTCAATGCACAATTTAGTATCATTAGTATTT TTTAATTTAAATTTCATTACTTTTTGAACCAATGATTTCTTAAATATTTTATTAAAAATAAATGGGAAAA CATAAGCGAATGGAAGAGGATTTTTAGCTAAATCAATTCTACGATCATTTATTAATCTCGCTTTTGGTTT TCAAGATATTGAACCATTAAGTCTTGGTTTGAATTCCAAAACATCAACATTATACTTTTCAGCATTTTTA ATCATATTTTCAATATAATAATACTTAATTAAAGTTATATCAGTATTAAATACTGTTATATACTCTCCCT TTGCTAATTTAAAGGCACTAAGAAGATTATATTGATAACTATTAGTCTTGGAATTATAAATAACTTGTAA TCTATTTTTAAATTTTTCAAAATAATTAGTTAATAATGAAATAATCTGTTTATTTTCAGATTCTTTTCCA TTTAAACATAAAATAATCTCAAAGTCATTTGAGGTTTGAGATAATAATGAATCTAAATATTTTTTTACAT TTTCAACGTTTTTTGTAACTAATGACACTAAAGATAATTTCATTTGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 518564-519214	Sequence	MCYN_0488 MCYN0488 RDD family protein (Protein is coded in complementary strand; from 519214 to 518564)
ORF.. 519138-519311	Sequence	MCYN_0489 MCYN0489 Hypothetical protein
ORF.. 519214-520242	Sequence	MCYN_0490 MCYN0490 Glycosyltransferase (Protein is coded in complementary strand; from 520242 to 519214)