Sequence retrieval

Genome: Vibrio parahaemolyticus BB22OP
Start position: 443740
End position: 444342
Length: 603

DNA sequence
>NC_019955, from 443739 to 444341 (603 bp); Vibrio parahaemolyticus BB22OP TTAGTCACTGATGGTAAACGTCTCGACATCTGGATCACTATCATCTTGTGTCTGTGTCTGTGTCTGTGTC TGTGTCTGTGTCTGTGTCTGTGTTGTCACACCGTCGCTAGGTAATTCGACTTTAACTGGTTCTGCGTCTC CGTATTGGAACCCTTTGATCACCTGTTTTACGCCAGAGATATTGCGCGAAATCTCCGTCGCGCGTTCACC TTGCTGAGCGGTTACATAGCCAAACAAAAATACGTCACCATCCTCTGTAATGACTTTGACTTTAACGCCA TTGAGGTCCGAGTCGGTTAATAGAGCCGATTTTACCTTGGTTGTGATCCAGCTGTCGTTGCTGATTTGCG TCACGCTCAATGGCTCTTTAGCCTTAATTTGGTTATGAATGGTTTTGACGCCATCAATGGCTCTAGCTCG TTGCTCAATTTGATTAATCAAATCTTGAGTTGGAGCTTGGCCCATTAAAACAACCGTACCATTGTACGAG CTCGCAACAACTCGAACATGCCCTTTAAACGGTTCTTTATTACCGATCGCTGCGACTTCAAATTCAATAT TGTTGTCTTGCCAGATCTGCTTCGTGGTGCGAGTATCTGTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 443740-444342	Sequence	VPBB_0427 - 21 kDa hemolysin precursor (Protein is coded in complementary strand; from 444342 to 443740)