Sequence retrieval

Genome: Escherichia coli PMV-1
Start position: 3139487
End position: 3139691
Length: 205

DNA sequence
>NC_022370, from 3139486 to 3139690 (205 bp); Escherichia coli PMV-1 GGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTT TTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTT TTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF... 3139647-3139874	Sequence	ECOPMV1_03067 ygdR_1 putative lipoprotein ygdR precursor (Protein is coded in complementary strand; from 3139874 to 3139647)
3139425-3139630	Sequence	ECOPMV1_03066 -