Sequence retrieval

Genome: Escherichia coli PMV-1
Start position: 543669
End position: 544904
Length: 1236

DNA sequence
>NC_022370, from 543668 to 544903 (1236 bp); Escherichia coli PMV-1 TTATTTCTGCCAGGCAAGTTGATAAAGCATGAGTGCCAACGTTTTGACCCCTTCGGCAAGGTCGGTAATA TTGGTGCGTTCCGCCGGGTTATGGCTGATCCCGTTGATGCTGGGAATGAAAATCATGCAGGTCGGCACGC GAGGCGCGAAAATTTGCGCGTCGTGCCCGGCACCACTGTGCATCACCCGGTAATTCAGTTTTTCGCTTTC ACACAATTCTGTCAGGGTGGCGACCAGCTCCTTATTCATCGGCACGGGTTCTTCGTCCATCCATAAATCG ATATCAATACCAATATCCATTTCATCGCAAATCGCCCGCATGTCGTTTTCTAACTGTTGGGTGAAATCGC GCAGCACGGTAGCGTCGGTATGACGACAATCAATGGTGAACGTGGTTTTACCCGGCACTACATTCACCGT ATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCTTTTTCGCTTTTTCGACA GACTGATGGCAAATGCGACTGAAAGCGTAAACCGTATCACGACGATAACCCATCGGCGTGGTGCCTGCAT GGTTTGATTCGCCATTCAGCGTCACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGA TTGCCCATTACTTTCCAGCACACAGCCCTGTTCGATATGCAGTTCGACAAAGGCTTTAATATCCTGACGC GGAGTTAGTGGGGCGTTCGGCAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGG CATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGAAAACATA CGGGAAGCGGCTGCCTTCTTCTTCTGCCATCGCCACCACTTCGACCGTACGTAACGGCGCGCCGTATTGC GTTTTCAGCCAGTCAATAGCCAGCCACGCCGCCAGCGCACCGAATTGCCCGTCGAGGTTACCGCCGTTAA CCACGGTATCGATATGCGAACCGCTCAGAACCACTTCCTGTGGATATTCGGTGCCACTCAGGCGACCGTA TAAATTCCCCACTTCATCGAAACGTGTTTCCAGCCCGCTTGCTGCCATTCTTTTTTTAAACTGCTGCTGG GTTTCCAGCCATTCCGGCGAATAAAGTAAACGGGTCATCCCACCCGCTGGGTCAGCGCCAAAAGAGGAAA GCCAGGGCAGCGTTTCTTCTATAGCTTGACGGAAATGTGTAATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 543669-544904	Sequence	ECOPMV1_00504 allC Allantoate amidohydrolase (Protein is coded in complementary strand; from 544904 to 543669)