Sequence retrieval

Genome: Rhizobium sp. IRBG74
Start position: 3901
End position: 4383
Length: 483

DNA sequence
>NC_022535, from 3900 to 4382 (483 bp); Rhizobium sp. IRBG74 TTAGTTCGGGACAGCCTGAACCTGAGCCTTTGCCTGTTCCTCGGCAACGCGCTGCGCAAACATCTGCGCA AAATCGATCGGATCGATCATGAGCGGCGGGAAACCGCCGTTGCGGGTAACGTCGGCAATGATCTGGCGGG CGAAGGGGAAGAGCAGGCGCGGGCACTCGATGAACAGCACCGGCAGCATGTGTTCCTGCGGGAAGCCGGC GATGCGGAAGACACCGCCATAGACCAGCTCGGCCGCAAACAGAACCTTATCGCCATCCTTGGCTTCGGCA TTCAGTGTCAGCACGACATCAAAATCCGAACCGGAAATCGGATTAGCATTGACGTTGACATTGATGTTGA TCGACGGCGCATTCTCACGGGCCTGCAGCGAACGAGGAGCACCCGGATTTTCAAAGGAAAGGTCCTTGAT GTACTGGGCAAGAATGTTGAGAGAAGGGCTAGCTGCGCCCTGTGCGCCATTTTCAGCGGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 3901-4383	Sequence	BN877_I0007 secB protein-export protein (Protein is coded in complementary strand; from 4383 to 3901)