Sequence retrieval

Genome: Rhizobium sp. IRBG74
Start position: 480491
End position: 482137
Length: 1647

DNA sequence
>NC_022535, from 480490 to 482136 (1647 bp); Rhizobium sp. IRBG74 TCATGGCTCTGCCTCCGGATTGCCGCCCGCCCGTGGCGCGGAAATGACGCCCTGGATATAGCTCGCGGGC AGATCGAGGATGCGCGCCGTTTCCTGCATGTAACGCATCACCGGCCCCGCCTGAACGGTCATGGCGAGTG TCAGGCCGAGAAGCAGCATGATGGGTGCGATTTCGATGACGAGCACGCGCGGCACGGTGCCTTCGATGGA GGCCCAGAAAGTACGGATGCCGGCACGGGTCATCGAGATCAGTGAAGCGAAGCCGGAAAAGACGATCAGG AAGACGATCCACCATGACAGGGTGGAGATGCTGTCATTGGCGCCGAGACCGGACGGATTGAGAATGGCCG ATAGCATCGAGAATTTGGCAATGAAGCCGGAAAGCGGCGGCAGGCCGGCGAGCAGGATGCCGCAGGCGGC AAAGCATGCGCCCAGCACGGCCATGGTGGCAGGCATGGTCACGCCGATTTCCTCTTCCTCGTCTTCTTCC TCGCCTTCGCCATAAGCCTCCATGGTTACGGCAAGAACGTTTGCGCCCGCATCCTGCCCGCGCTCGACAA GTTCGATGAGCATGAAGAAGGCACTGATGGTGAGGGTGGAGCTGACAAGGTAATAAAGTGCGCCGGCCGC CACCGTGGGATTGCCGGTACCCATGGCTGCAAGCAGCGTTCCCGATGAGACCAGAACCGAAAAGCCGGCA AGCCGTCCCAGCGCCTGCGACGCCAGAACCCCGATGGCACCGAAGATGATGGTGGCGATGCCGCCGACCA GCAGCGCATCGTGGCCGAAACCGGCCGATTCCCCGGCCGTCTGGCCGAACAGCAGGAAGGAGAGGCGGGC GATGACATAAATGCCGACCTTGCTCATGATCGCGAATAGCCCGCCGACCGGTGCAGATGCCGCACCATAG GCGGAGGGCAGCCAGAAATTCAGCGGCCACATGCCGGCCTTGACCAGAAACGCGATGCCGAGGATGGCCG AGCCGGTTTCCAGCAGCATGCGCTGGTCGGGGTTCAGCCCCTCTATCCGGTGGGCGAGATCACCCATGTT CAGCGTGCCCGTCACGCCATAGATCAGACTGACGCCAATCAGGAAAAACAAGGCGGCAACGAGGTTGATG GCGATGTAATGCAGGCCCGCCTTGACGCGTTGCTGGCCGGAGCCATGCAGCAGCAGCCCGTAAGATGCCG CCAGCATCACTTCGAAGAATACGAAAAGGTTGAAGAGGTCGCCGGTCAGGAACGCGCCGTTGACGCCCAT CAGCATCATCTGGAACAGCGAATGGAAATGGGCGCCCGCCTTGTGCCATTTCGCCATCGAATAGATGAGC GAGGGAATGGCGAGCAATGCGGTCAACAGGACCATCAGGCCCGACAGGCGGTCGGCGACGAGCACGATGC CGAAGGGTGCCGGCCAGTTGCCGAGGAGATAGACGCCGCTCGAAACCACAGCAGCGATCTCGGTATTAAC GGTTGAGTTCACCTCGCGGAACAGCAGAATCGCAACCACCAGCAGCAGCACCGTGGAGGTGAAGCTGATC GCGGCTTTGGCCACACGCTGGCGCTCGTCAAAAAACAGCAGCAGCGCGGCGGTAATGAGCGGCAGCAGGA TGGGTGCGATGACGATGTGGAAGGGGAACGACATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 480006-480494	Sequence	BN877_I0486 phaE multisubunit K+/H+ antiporter subunit E (Protein is coded in complementary strand; from 480494 to 480006)
ORF.. 480491-482137	Sequence	BN877_I0487 phaD multisubunit K+/H+ antiporter subunit D (Protein is coded in complementary strand; from 482137 to 480491)
ORF... 482134-482469	Sequence	BN877_I0488 phaC multisubunit K+/H+ antiporter subunit C (Protein is coded in complementary strand; from 482469 to 482134)