Sequence retrieval

Genome: Rhizobium sp. IRBG74
Start position: 5162
End position: 5860
Length: 699

DNA sequence
>NC_022535, from 5161 to 5859 (699 bp); Rhizobium sp. IRBG74 ATGGGCTTCAGCGACTTTATCACATTGTTTTTTCTCGTTGCAGCGGTGCTGATCTTCTTTCAGCTGCGCA GCGTCCTTGGTCGTCGCACGGGCAATGAAAAGCCGCCTTTTGATCCTTATAGCCCGCGCGATGTCGCGAA GGGTCCCGTCACGGATGATAACAAGGTCGTGACACTGCCGAAGCGCGCCGAGGCGGAAGATGAAAACCGC TTCGCCGAGGTGGATGCACTGGCGCCTGCAGACAGCGCACTCAATGCCTCCCTGCGTGAGGTGATGACGA AGGATCCGTCCTTCCGCCCGAAGGAATTCCTGAACGGCGCCCGCATGGCCTACGAGATGATCGTGATGGG TTTTGCCGATGGCGACCGCAAGACGCTCAAGAACCTGCTGTCCAAAGAGGTGTTTGAAGGTTTCGACGCA GCGATTTCCGAAAGGGAAGGCCGCGGCGAGGTCGTGAAATCGACTTTTGTTGGCATTGAGAAAGCCGATA TCACCCAGGCGGGTATTCGCGATTCCGAGGTGCAGATAACGCTCCGGATCGTCAGCCAGCTCATCTCGGC GACCTATGACAAGGATGGCAAGCTGGTCGATGGTGATCCGGATGCGGTTGCGGAGGTGGATGACATCTGG ACCTTCTCGCGCGATATCCGCTCACGCGATCCGAACTGGAAGCTGATCGCCACCGAATCCGAGCAATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5162-5860	Sequence	BN877_I0009 - hypothetical protein
ORF... 5857-6969	Sequence	BN877_I0010 - Membrane-bound lytic murein transglycosylase