Suggestion of primers for restricted AP-PCR (resAP-PCR)

This is a suggestion tool for selection of the set of three oligonucleotides for resAP-PCR.

To perform this technique, the genomic DNA of the selected microorganism must be digested with HaeIII (GG|CC) or AluI (AG'CT) prior to amplificacion with three 9 mer oligonucleotides. The oligonucleotides has been selected based on genome sequence of each strain, and their usage to genotype member of the same species is likely to yield comparable bands patterns in agarose gels. More

This technique is only available for organisms with apropiate C+G content (around 50% or higher).

Citing this site