Restriction enzyme digest of DNA
with commercially available restriction enzymes

Beta version: correct behaviour in being checked

Paste the sequence in the textbox as plain text, fasta or upload sequence.

  • Upload file:
    The file must be a text file with code in plain text or in fasta format (<3 MB)
  • No sequences available in the server

Display of Results: Table Tabulated text
Show sequence(s) on top
When two or more input sequences are searched:
Show only endonucleases showing different restriction patterns for searched sequences.
Settings: Default Change
Select specific endonucleases: Select

Version: 1.20131212 , based in REBASE version 312
This service recognizes 269 different cleavage patterns (from all 620 commercially available endonucleases).
This tool was described by San Millán et al, 2013 (DOI: 10.1186/1756-0500-6-513).
Copy and paste PHP script is freely available at
Restriction Home.