In silico Double Digestion Fingerprinting (DDF)



This simulation tool for Double Digestion Fingerprinting technique allows  AFLP-PCR and SRF techniques to be simulated, and also novel aprouches. We have included a few examples to show the us of this tool.

  link Simulation of AFLP-PCR experiment
  link Simulation of Subtracted Restriction Fingerprinting (SRF) experiment
  link Simulation of AFLP-PCR like experiment when using one non-palindromic endonuclease
  link Simulation of AFLP-PCR like experiment when using a non-palindromic endonuclease
 with degenerated (N) nucleotides
  link Simulation of AFLP-PCR like experiment when using only one endonuclease
  link Simulation of Double Digestion Fingerprinting experiment and detection of bands with labelled adaptors

Have you tried a different approuch not listed above? Please let us know.

Frequency of recognition site for commercial endonucleases in sequenced bacterial genomes may be checked here.

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