The aim of this tool is to provide the number of fragments we may get when a DNA sequence is digested with restriction enzymes. As the results are focused in fragments, overlapping recognition sequences are not accounted.

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Minimum recognition size for each restriction enzyme 4 5 6 7 8 Type of restriction enzyme All Blunt ends Overhang end Commercial source All Amersham Pharmacia Biotech Minotech Biotechnology Stratagene Fermentas AB Qbiogene American Allied Biochemical, Inc. SibEnzyme Ltd.\n Nippon Gene Co., Ltd. Takara Shuzo Co. Ltd. Roche Applied Science New England Biolabs Toyobo Biochemicals Megabase Research Products CHIMERx Promega Corporation Sigma Chemical Corporation Bangalore Genei MRC-Holland EURx Ltd. Only restriction enzymes with known bases (no N,R,Y...) Include Type IIb restriction enzymes (Two cleaves per recognition sequence) Include Type IIs restriction enzymes (Non-palindromic and cleavage outside of the recognition site)

DNA sequences can be pasted as shown or just as a one line string (number, spaces and line feeds will be ignored)

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