DNA to protein translation tool
This tool works similarly to other ones available online or programs
allowing this feature. Genetic codes used in this service are those ones
compiled by Andrzej
(Anjay) Elzanowski and Jim Ostell.
DNA sequence may be added as shown in the example sequence or in any
able to perform small tasks as for example tiding up the sequence and
getting reverse or complement sequences.
Translation to protein will be performed by using one of the predefined
genetic codes, or by using custom genetic code.
Minimum size of protein sequence for Open Reading Frames (ORF) is
customizable, and they can be trimmed to MET-to-Stop.
Showing translation alignment is optional, and aminoacids will be
displaied as a 1-letter aminoacids code.
After translation, in the response page ORFs are shown as arrow. In
order to check ORFs represented by those arrows, click on them and a new
browser window will be opened showing in red letters the DNA sequence
corresponding to that specific ORF and translated protein. This feature
How to use custom
The genetic code used to translate a sequence into protein may be
This service allows introducing the genetic code as a string, where
each character corresponds to one aminoacid and asteriscs represents
termination codes. In the example bellow is shown the standard genetic
code and the corresponding triplets.
In the first line, the first character ("F")
represents Phenylalanine,which is encoded by the triplet TTT (first
character of "Base1",
-- Base1 TTTTTTTTTTTTTTTTCCCCCCCCCCCCCCCCAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGG
-- Base2 TTTTCCCCAAAAGGGGTTTTCCCCAAAAGGGGTTTTCCCCAAAAGGGGTTTTCCCCAAAAGGGG
-- Base3 TCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAG
first character of "Base2" and first character of "Base3")
The eleventh character ("*") represents a termination code, which is
encoded by the triplet TAA.
The custom genetic code provided must be 64 characters long.
Correspondence between characters and aminoacids may follow the system
used in this service or may be different, but it will be always case
Methionine as a initiation code
When searching "ORFs trimmed to MET-to-Stop", they will be shown the
longest ORFs available (from methionine to Stop), so that within the
ORF there may be several methionines, as for example in the
aminoacid secuence bellow:
1-letter aminoacid codes
A alanine P proline
B aspartate or asparagine Q glutamine
C cysteine R arginine
D aspartate S serine
E glutamate T threonine
F phenylalanine U selenocysteine
G glycine V valine
H histidine W tryptophan
I isoleucine Y tyrosine
K lysine Z glutamate or glutamine
L leucine X any
M methionine * translation stop
N asparagine - gap of indeterminate length